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Microinjection of DplUSE RNA causes a decrease in GFP expression in transgenic GFP-DplUSE animals. ( A ) Representative image of a <t>5</t> <t>dpf</t> GFP-DplUSE transgenic line. ( B ) Confocal images of transgenic GFP-DplUSE zebrafish, stained with the nuclear marker <t>DAPI,</t> showing GFP expression in the notochord and muscle (scale bar = 10 μm). ( C ) Representative image of GFP expression in transgenic GFP-DplUSE zebrafish embryos microinjected with DplUSE RNA or DplUSEmt RNA at 5 dpf. Images were acquired using Leica M205. ( D ) Quantification of GFP expression in notochord ( n = 75 Non Injected (NI); n = 64 DplUSE; n = 54 DplUSEmt) and ( E ) in muscle ( n = 70 NI; n = 53 DplUSE; n = 54 DplUSEmt). ( F ) Quantification of the percentage of transgenic GFP-DplUSE embryos that showed a patchy expression of GFP in the notochord (36.5%, n = 73 NI; 73%, n = 69 DplUSE; 50%, n = 59 DplUSEmt). The dashed lines represent the regions where GFP was quantified. Statistical significance was determined by χ2 test with Fisher correction or by two-tailed unpaired t-test. *** P < .001; ** P < .01; * P < .05; ns P > .05. (scale bar = 100 μm).
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Microinjection of DplUSE RNA causes a decrease in GFP expression in transgenic GFP-DplUSE animals. ( A ) Representative image of a 5 dpf GFP-DplUSE transgenic line. ( B ) Confocal images of transgenic GFP-DplUSE zebrafish, stained with the nuclear marker DAPI, showing GFP expression in the notochord and muscle (scale bar = 10 μm). ( C ) Representative image of GFP expression in transgenic GFP-DplUSE zebrafish embryos microinjected with DplUSE RNA or DplUSEmt RNA at 5 dpf. Images were acquired using Leica M205. ( D ) Quantification of GFP expression in notochord ( n = 75 Non Injected (NI); n = 64 DplUSE; n = 54 DplUSEmt) and ( E ) in muscle ( n = 70 NI; n = 53 DplUSE; n = 54 DplUSEmt). ( F ) Quantification of the percentage of transgenic GFP-DplUSE embryos that showed a patchy expression of GFP in the notochord (36.5%, n = 73 NI; 73%, n = 69 DplUSE; 50%, n = 59 DplUSEmt). The dashed lines represent the regions where GFP was quantified. Statistical significance was determined by χ2 test with Fisher correction or by two-tailed unpaired t-test. *** P < .001; ** P < .01; * P < .05; ns P > .05. (scale bar = 100 μm).

Journal: Nucleic Acids Research

Article Title: A conserved 3′UTR short motif regulates gene expression in vertebrates

doi: 10.1093/nar/gkaf1340

Figure Lengend Snippet: Microinjection of DplUSE RNA causes a decrease in GFP expression in transgenic GFP-DplUSE animals. ( A ) Representative image of a 5 dpf GFP-DplUSE transgenic line. ( B ) Confocal images of transgenic GFP-DplUSE zebrafish, stained with the nuclear marker DAPI, showing GFP expression in the notochord and muscle (scale bar = 10 μm). ( C ) Representative image of GFP expression in transgenic GFP-DplUSE zebrafish embryos microinjected with DplUSE RNA or DplUSEmt RNA at 5 dpf. Images were acquired using Leica M205. ( D ) Quantification of GFP expression in notochord ( n = 75 Non Injected (NI); n = 64 DplUSE; n = 54 DplUSEmt) and ( E ) in muscle ( n = 70 NI; n = 53 DplUSE; n = 54 DplUSEmt). ( F ) Quantification of the percentage of transgenic GFP-DplUSE embryos that showed a patchy expression of GFP in the notochord (36.5%, n = 73 NI; 73%, n = 69 DplUSE; 50%, n = 59 DplUSEmt). The dashed lines represent the regions where GFP was quantified. Statistical significance was determined by χ2 test with Fisher correction or by two-tailed unpaired t-test. *** P < .001; ** P < .01; * P < .05; ns P > .05. (scale bar = 100 μm).

Article Snippet: Embryos were raised until they reached the 4 dpf larval stage, fixed, and stained with DAPI [PUREBLUTM DAPI (1351303; Bio-Rad)].

Techniques: Microinjection, Expressing, Transgenic Assay, Staining, Marker, Injection, Two Tailed Test